Acute pancreatitis (AP) is a complex disorder with gallstones being the most common underlying cause. Anatomical variations of gallbladder, cystic duct (CD), common bile duct and main pancreatic duct and their courses and interactions with each other have been studied and shown to be related to development of AP in various studies. With this study, we aimed to investigate the relationship between biliopancreatic tree anatomy and acute gallstone pancreatitis.